Utilization of Nipa Palm (Nypa fruticans) Leaf Extract as a Natural Preservative for Salted Three Spot Gourami (Trichopodus trichopterus)
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Abstract
Background: Salted three-spot gourami (Trichopodus trichopterus) is a traditional fish product that is susceptible to microbial spoilage and quality deterioration during storage. The use of plant-based bio-preservatives has been explored as an alternative to synthetic additives to improve product safety and shelf life.
Aims: This study aimed to assess the effectiveness of nipa palm (Nypa fruticans) leaf extract at different concentrations as a natural preservative for salted three-spot gourami.
Methods: The experiment was arranged in a Completely Randomized Design (CRD) with four treatments (0%, 10%, 15%, and 20%) and three replications. Parameters observed included moisture content, Total Plate Count (TPC), and organoleptic attributes (appearance, aroma, and texture).
Results: The application of nipa palm leaf extract significantly (P < 0.05) affected moisture content, microbial growth, and sensory quality during storage. The 15% treatment (P2) produced the lowest moisture content throughout storage, reaching 19.83% on day 30. Microbial counts in all extract-treated samples remained within the maximum permissible limit of the Indonesian National Standard (≤1 × 10⁵ CFU/g), with the 20% treatment (P3) showing the lowest microbial load (1.0 × 10⁵ CFU/g) on day 30. Sensory evaluation indicated that extract-treated samples maintained acceptable appearance, aroma, and texture throughout storage, with better sensory stability than the untreated control.
Conclusion: Nipa palm leaf extract effectively improved the storage quality of salted three-spot gourami. Although the 15% concentration (P2) achieved the greatest moisture reduction, the 20% concentration (P3) is recommended for practical application because it provided the strongest microbial inhibition while maintaining acceptable sensory quality during 30 days of storage.
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Copyright (c) 2026 Agustin Maharani Khairunisa, Riya Liuhartana Nasyiruddin Fuhrmann, Rih Laksmi Utpalasari, Zhulian Hikmah Hasibuan, RR. Dyah Paramitha Mentari, Steven Suryoprabowo, Muhammad Shoaib

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References
Birie, S., Mingist, M., Kibret, M., Atlog, T. Y., Geremew, H., & Getnet, B. (2024). Proximate Composition, Microbiological Quality and Safety of Raw and Open Sun‐Dried Fish Products in Lake Tana, Ethiopia. Food Science & Nutrition, 13. https://doi.org/10.1002/fsn3.4671
BSN. (2006a). SNI-01-2354.2-2006 Standar Nasional Indonesia Cara uji kimia-Bagian 2: Penentuan kadar air pada produk perikanan.
BSN. (2006b). SNI 01-2332.3-2006 Standar Nasional Indonesia Cara uji mikrobiologi-Bagian 3: Penentuan angka lempeng total (ALT) pada produk perikanan.
BSN. (2023). SNI 8273:2023 Standar Nasional Indonesia Ikan asin kering. https://www.bsn.go.id
BSN. (2025). SNI 2346:2025 Standar Nasional Indonesia Pedoman pengujian sensori pada hasil perikanan. https://www.bsn.go.id
Daglia, M. (2012). Polyphenols as antimicrobial agents. Current Opinion in Biotechnology, 23(2), 174–181. https://doi.org/10.1016/J.COPBIO.2011.08.007
Damopolii, Y., Maspeke, N. S., Une, S., Program, ), Pangan, S. T., & Gorontalo, U. N. (2024). Pemanfaatan Pengawet Alami Ekstrak Daun Kemangi (Ocimum Basilicum) Sebagai Penghambat Pertumbuhan Mikroorganisme pada Ikan Kembung (Rastrelliger spp) Asin Kering. Jambura Journal of Food Technology, 6(1), 1–17. https://doi.org/10.37905/JJFT.V6I1.11039
De Rossi, L., Rocchetti, G., Lucini, L., & Rebecchi, A. (2025). Antimicrobial Potential of Polyphenols: Mechanisms of Action and Microbial Responses—A Narrative Review. Antioxidants, 14. https://doi.org/10.3390/antiox14020200
Dewi, A. K., Yulianto, A. N., & Setiyabudi, L. (2023). Formulasi dan Uji Antibakteri Sabun Cair Ekstrak Daun Nipah (Nypa fruticans) terhadap Bakteri Staphylococcus aureus. Sains Indonesiana, 1(1), 100–108. https://sainsindonesiana.id/index.php/sainsindonesiana/article/view/14
FAO. (2022). The State of World Fisheries and Aquaculture 2022. Towards Blue Transformation. In The State of World Fisheries and Aquaculture 2022. Towards Blue Transformation. FAO. https://doi.org/10.4060/CC0461EN
Haetami, K., Karlina, L., Kunci, K., Asin, I., Segar, I., & Tradisional, P. (2024). Formalin Content Test in Samples of Salted Fish and Fresh Fish Sold in Traditional Markets. PharmaCine : Journal of Pharmacy, Medical and Health Science, 5(1), 48–54. https://doi.org/10.35706/PC.V5I1.11339
Hanafiah, K. A. (2010). Rancangan Percobaan; Teori dan Aplikasi (3rd ed.). Rajawali Pers.
Indrastuti, N. A., Wulandari, N., Palupi, N. S., Studi, P., Pangan, I., Ilmu, D., & Pangan, T. (2019). Profile of Salted Fish Processing in Pengolahan Hasil Perikanan (PHPT) Muara Angke. Jurnal Pengolahan Hasil Perikanan Indonesia, 22(2), 218–228. https://doi.org/10.17844/JPHPI.V22I2.27363
Jusmaldi, J., Dianingrum, A. R., & Hariani, N. (2021). The growth pattern and condition factors of three spot gourami Trichopodus trichopterus (Pallas, 1770) from the Lempake Dam, East Kalimantan. Jurnal Iktiologi Indonesia, 21(3), 215–233. https://doi.org/10.32491/JII.V21I3.588
Li, Y., Ou, J., Huang, C., Liu, F., Ou, S., & Zheng, J. (2023). Chemistry of formation and elimination of formaldehyde in foods. Trends in Food Science & Technology, 139, 104134. https://doi.org/10.1016/J.TIFS.2023.104134
Lobiuc, A., Pavăl, N.-E., Mangalagiu, I., Gheorghita, R., Teliban, G., Amăriucăi-Mantu, D., & Stoleru, V. (2023). Future Antimicrobials: Natural and Functionalized Phenolics. Molecules, 28. https://doi.org/10.3390/molecules28031114
Narzary, Y., Das, S., Goyal, A. K., Lam, S. S., Sarma, H., & Sharma, D. (2021). Fermented fish products in South and Southeast Asian cuisine: indigenous technology processes, nutrient composition, and cultural significance. Journal of Ethnic Foods 2021 8:1, 8(1), 33-. https://doi.org/10.1186/S42779-021-00109-0
Nosić, M. (2026). Biochemical and Microbiological Safety Risks in Salted Fish Products: A Review. Food and Drug Safety, 3(1), 88–102. https://doi.org/10.55121/FDS.V3I1.1082
Oulahal, N., & Degraeve, P. (2022). Phenolic-Rich Plant Extracts With Antimicrobial Activity: An Alternative to Food Preservatives and Biocides? Frontiers in Microbiology, 12, 753518. https://doi.org/10.3389/FMICB.2021.753518/FULL
Pereira, A. G., Perez-Vazquez, A., Barciela, P., Jorge, A. O. S., Yuksek, E. N., & Prieto, M. A. (2026). Natural Antimicrobials from Plants Used as Food Preservatives. Foods 2026, Vol. 15, Page 1309, 15(8), 1309. https://doi.org/10.3390/FOODS15081309
Plaskova, A., & Mlcek, J. (2023). New insights of the application of water or ethanol-water plant extract rich in active compounds in food. Frontiers in Nutrition, 10, 1118761. https://doi.org/10.3389/FNUT.2023.1118761/FULL
Presenza, L., Ferraz Teixeira, B., Antunes Galvão, J., & Maria Ferreira de Souza Vieira, T. (2023). Technological strategies for the use of plant-derived compounds in the preservation of fish products. Food Chemistry, 419, 136069. https://doi.org/10.1016/J.FOODCHEM.2023.136069
Puspitasari, F., Aisyah, S., Wilianti, S. A., Albarah, K. S., & Adawyah, R. (2021). Pengaruh Penambahan Garam pada Perubahan Karakteristik Kimia dan Pertumbuhan Bakteri pada Ikan Sepat Rawa (Trichogaster trichopterus): The Effect of Salt Addition on Chemical Characteristics of and Bacterial Growth on Three Spot Gourami (Trichogaster tri…. Jurnal Pengolahan Hasil Perikanan Indonesia, 24(1), 113–121. https://doi.org/10.17844/JPHPI.V24I1.32622
Quoc, L. P. T., Anh, T. T. M., Phuong, L. B. B., Quyen, P. T., & Hao, P. M. (2025). Phytochemicals, antimicrobial and antioxidant properties, and potential applications of Nypa fruticans Wurmb.: An updated review. Food Science and Preservation, 32(6), 996–1007. https://doi.org/10.11002/FSP.2025.32.6.996
Sumartini, & Purnama, R. (2021). The Ekstrak Daun Mangrove (Sonneratia caseolaris) sebagai Pengawet Alami Ikan Tongkol (Euthynnus affinis) Selama Penyimpanan. Jurnal Airaha, 10(01), 109–122. https://doi.org/10.15578/JA.V10I01.250
Sun, X., Yang, C., Zhang, W., Zheng, J., Ou, J., & Ou, S. (2025). Toxicity of formaldehyde, and its role in the formation of harmful and aromatic compounds during food processing. Food Chemistry: X, 25, 102225. https://doi.org/10.1016/J.FOCHX.2025.102225
Tahiluddin, A. B., Maribao, I. P., Amlani, M. Q., & Sarri, J. H. (2022). A Review on Spoilage Microorganisms in Fresh and Processed Aquatic Food Products. Food Bulletin, 1(1), 21–36. https://doi.org/10.29329/FOODB.2022.495.05
Utami, T. F. Y. (2022). Evaluasi Sediaan Krim dan Uji Toksisitas Akut Ekstrak Daun Nipah (Nypa fruticans Wurmb) Daerah Cilacap Dengan Fase Minyak VCO Sebagai Kandidat Antibakteri. Indonesian Journal of Pharmacy and Natural Product, 5(1), 82–90. https://doi.org/10.35473/IJPNP.V5I1.1596
Agustin Maharani Khairunisa